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Updated: Mar 2, 2026
13:43
Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
Published on: June 24, 2013
14.6K
A fully integrated, three-dimensional fluorescence to electron microscopy correlative workflow
Claudia S López1, Cedric Bouchet-Marquis2, Christopher P Arthur3
1Oregon Health and Sciences University, Portland, OR, United States.
Methods in Cell Biology
|May 23, 2017
Summary
Correlative light and electron microscopy (CLEM) overcomes fluorescence microscopy limitations. This study demonstrates an integrated CLEM workflow for enhanced cellular structure and protein localization analysis in breast cancer cells.
Area of Science:
- Cell Biology
- Microscopy
- Biotechnology
Background:
- Fluorescence microscopy offers specific labeling but has resolution limits and poor contrast.
- Studying cellular structure and protein localization is challenging with traditional microscopy.
- Correlative Light and Electron Microscopy (CLEM) integrates multiple imaging modalities.
Purpose of the Study:
- To demonstrate an integrated correlative light and electron microscopy (CLEM) workflow.
- To showcase the FEI CorrSight with MAPS for reliable and rapid CLEM data correlation.
- To apply the integrated CLEM workflow to fluorescently tagged MCF7 breast cancer cells.
Main Methods:
- Utilizing an integrated CLEM workflow instrument (FEI CorrSight with MAPS).
- Correlating three-dimensional time-lapse confocal fluorescence microscopy data.
- Correlating three-dimensional focused ion beam-scanning electron microscopy data.
Main Results:
- Demonstration of a complete, integrated CLEM workflow.
- Successful correlation of fluorescence and electron microscopy data.
- Application of the workflow to MCF7 breast cancer cells.
Conclusions:
- Integrated CLEM workflows provide a reliable, reproducible, and faster approach for cellular imaging.
- This method enhances the study of cellular structure and protein localization.
- The demonstrated workflow is applicable to cancer cell research.
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