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Recent advances in capillary ultrahigh pressure liquid chromatography
Laura E Blue1, Edward G Franklin2, Justin M Godinho3
1Process Development, Amgen Inc., Thousand Oaks, CA 91320, USA.
Journal of Chromatography. A
|June 11, 2017
Summary
Capillary ultrahigh pressure liquid chromatography (UHPLC) has advanced significantly since 2010, enhancing separation performance for complex mixtures. Recent innovations in columns, stationary phases, and instrumentation are driving new applications, especially in proteomics.
Area of Science:
- Analytical Chemistry
- Chromatography
- Separation Science
Background:
- Capillary ultrahigh pressure liquid chromatography (UHPLC) is a powerful technique for analyzing complex mixtures.
- Significant advancements have been made in UHPLC technology over the past decade.
Purpose of the Study:
- To review recent advances in capillary UHPLC performance since 2010.
- To highlight improvements in column preparation, stationary phases, and instrumentation.
- To discuss the impact on proteomics and future applications in complex sample separation.
Main Methods:
- Review of literature focusing on capillary UHPLC developments since 2010.
- Analysis of improvements in capillary column preparation techniques.
- Examination of new stationary phases, phase supports, and associated instrumentation.
Main Results:
- Development of capillary columns with unprecedented performance.
- Introduction of novel stationary phases and phase supports for capillary UHPLC.
- Instrumentation advancements to mitigate extra-column effects and preserve column efficiency.
Conclusions:
- Recent innovations have substantially increased the performance of capillary UHPLC.
- These advancements are crucial for the analysis of complex samples, particularly in proteomics.
- Capillary UHPLC is poised for continued impact and application in challenging separation tasks.
Keywords:
Capillary LCLC columnsProteomicsStationary phasesUltrahigh pressure liquid chromatographynanoLCMore Related Videos
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