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Microbicidal Effects of Stored Aqueous Ozone Solution Generated by Nano-bubble Technology
Mineaki Seki1, Tatsuya Ishikawa2, Hiroshi Terada2
1Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan seki@nupals.ac.jp.
In Vivo (Athens, Greece)
|June 28, 2017
Summary
Nano-bubble ozone water (NBOW) retains its microbicidal activity for over a year when stored frozen, offering a potent and safe disinfectant alternative. This innovative ozone water formulation is also resistant to freeze-thawing cycles.
Area of Science:
- Microbiology
- Material Science
- Environmental Science
Background:
- Clinically used disinfectants can cause skin irritation and leave harmful residues.
- Ozone water is a unique disinfectant that decomposes into oxygen, posing no risk to skin cells.
- Conventional ozone water loses its sanitizing efficacy rapidly, limiting its practical application.
Purpose of the Study:
- To evaluate the long-term microbicidal efficacy of nano-bubble ozone water (NBOW).
- To assess the stability and effectiveness of NBOW after prolonged storage.
- To determine the impact of storage conditions on NBOW's sanitizing properties.
Main Methods:
- Ozone concentration in NBOW was monitored using redox potential measurements.
- Microbicidal activity was quantified through colony formation assays.
- Bacteria, including disinfectant-resistant Mycobacterium smegmatis, were incubated with NBOW for specified durations.
Main Results:
- NBOW stored at 4°C for 1 year lost its microbicidal activity.
- NBOW stored frozen retained significant microbicidal activity after 1 year.
- NBOW demonstrated resistance to freeze-thawing cycles and effectively killed Mycobacterium smegmatis within 15 minutes.
Conclusions:
- Frozen storage preserves the microbicidal efficacy of NBOW for over a year.
- NBOW exhibits remarkable stability and resistance to freeze-thawing.
- NBOW presents a promising, effective, and safe alternative disinfectant for various applications.
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