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Changed enzyme activities in rat kidney during ischemia.
The Journal of Surgical Research
|November 1, 1985
Summary
Kidney warm ischemia alters specific enzyme activities, particularly plasma membrane enzymes like ATPase, within 30 minutes. This enzymatic analysis aids in evaluating protective agents against kidney ischemia.
Area of Science:
- Biochemistry
- Nephrology
- Cell Biology
Background:
- Kidney ischemia can lead to cellular damage and organ dysfunction.
- Understanding the temporal changes in enzyme activity during ischemia is crucial for assessing kidney injury.
Purpose of the Study:
- To investigate the impact of varying durations of warm ischemia on marker enzyme activities in rat kidney cortex.
- To identify specific enzymes that are sensitive indicators of ischemic damage.
Main Methods:
- Analysis of marker enzyme activities in rat kidney cortex homogenates subjected to 15, 30, 60, and 90 minutes of warm ischemia.
- Enzymes analyzed included lactate dehydrogenase, alanine aminopeptidase, N-acetyl-beta-D-glucosaminidase, succinate-cytochrome c reductase, ATPase, 5'-nucleotidase, K-Mg-nitrophenylphosphatase, glucose-6-phosphatase, and NADPH-cytochrome c reductase.
Main Results:
- Lactate dehydrogenase, alanine aminopeptidase, N-acetyl-beta-D-glucosaminidase, and succinate-cytochrome c reductase activities remained unchanged.
- ATPase, 5'-nucleotidase, and K-Mg-nitrophenylphosphatase activities declined within 30 minutes of ischemia.
- Microsomal enzymes glucose-6-phosphatase and NADPH-cytochrome c reductase decreased after 60 minutes of ischemia.
Conclusions:
- Early decreases in ATPase and plasma membrane enzyme activities suggest membrane alterations during ischemia.
- Enzyme activity profiling provides a valuable method for evaluating therapeutic strategies against kidney ischemic injury.