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Updated: Feb 25, 2026

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
Venipuncture Procedures: Sources of Human Peripheral Blood Cells, Serum, and Plasma
Barbara E Bierer1, Warren Strober2
1Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
Abstract:
Peripheral blood is the chief source of mononuclear cells (lymphocytes and monocytes) for immunologic studies of humans. This appendix presents protocols for obtaining blood by simple venipuncture when relatively small amounts of blood (10 to 100 ml) are necessary or by lymphapheresis when large amounts (300 to 5000 ml) are necessary. Cells collected by these procedures can be further separated by techniques described in Chapter 7. © 2017 by John Wiley & Sons, Inc.
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