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Updated: Feb 25, 2026
Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
Published on: July 22, 2013
Label Free Poly(2,5-dimethoxyaniline)-Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B₁
Milua Masikini1, Avril R Williams2, Christopher E Sunday3
1SensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa. mmasikini@uwc.ac.za.
Abstract:
An impedimetric immunosensor for fumonisin B₁ (FB₁) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The preparation of the FB₁ immunosensor involved the drop-coating of a bovine serum albumin mixture of the anti-fumonisin antibody (anti-Fms) onto the composite polymer-modified GCE. The electrochemical impedance spectroscopy (EIS) responses of the FB₁ immunosensor (GCE/PDMA-MWCNT/anti-Fms) have a linear range of 7 to 49 ng·L-1, and the corresponding sensitivity and detection limits are 0.272 kΩ L·ng-1 and 3.8 pg·L-1, respectively. The limit of detection of the immunosensor for certified corn sample (i.e., certified reference material) is 0.014 ppm FB₁, which is in excellent agreement with the value published by the vendors and significantly more accurate than that obtained with enzyme-linked immunosorbent assay (ELISA).
