A new conjugated polymer-based combination probe for ATP detection using a multisite-binding and FRET strategy

Area of Science:

• Biochemistry
• Materials Science
• Analytical Chemistry

Background:

• Adenosine triphosphate (ATP) is a crucial molecule in cellular energy transfer and signaling.
• Accurate detection of ATP is vital for understanding biological processes and diagnosing diseases.
• Existing ATP detection methods often suffer from low selectivity or complex procedures.

Purpose of the Study:

• To develop a novel conjugated polymer-based probe for sensitive and selective detection of ATP.
• To utilize a combination strategy involving multisite-binding and fluorescence resonance energy transfer (FRET).
• To demonstrate the probe's ability to discriminate ATP from common interfering substances.

Main Methods:

• Construction of a conjugated polymer-based ratiometric probe.
• Application of a multisite-binding strategy for enhanced ATP recognition.
• Implementation of fluorescence resonance energy transfer (FRET) for signal transduction.
• Testing the probe's selectivity against various interfering molecules like ADP, AMP, and nucleobases.

Main Results:

• The developed probe exhibited high sensitivity and selectivity for ATP detection.
• The ratiometric detection strategy provided reliable signal output.
• The probe successfully differentiated ATP from structurally similar compounds, including ADP, AMP, other nucleoside polyphosphates, and nucleobases.
• The detection method was found to be rapid and efficient.

Conclusions:

• A new conjugated polymer-based ratiometric probe has been successfully developed for ATP detection.
• The probe leverages a combination of multisite-binding and FRET for enhanced performance.
• The method offers a rapid, highly selective, and reliable approach for discriminating ATP from common interferents.