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Visible-Light-Responsive Surfaces for Efficient, Noninvasive Cell Sheet Harvesting.

Xiaozhao Wang1, Cai Yao2, Wenjian Weng1

  • 1School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University , Hangzhou 310027, China.

ACS Applied Materials & Interfaces
|August 11, 2017
PubMed
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Researchers developed a new method for harvesting cell sheets using visible light and silicon wafers. This technique allows for rapid, gentle detachment of cells, preserving their viability for regenerative medicine applications.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Surface Chemistry

Background:

  • Effective regulation of cell-surface interactions is crucial for advancing cell-based therapies and regenerative medicine.
  • Current methods for cell sheet harvesting can be time-consuming and may compromise cell viability and function.
  • Developing non-invasive and efficient cell harvesting techniques is essential for clinical translation.

Purpose of the Study:

  • To introduce a novel method for visible-light-induced cell sheet harvesting using silicon wafers with a p/n junction (Si(p/n)).
  • To investigate the mechanism of cell sheet detachment and the role of protein-material interactions.
  • To demonstrate the preservation of cell viability and function after harvesting.

Main Methods:

  • Utilizing silicon wafers with a p/n junction (Si(p/n)) as a substrate for cell culture.
Keywords:
MD simulationcell sheetphotovoltaic effectprotein desorptionregenerative medicinesiliconvisible light

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  • Applying visible-light illumination to induce cell sheet detachment.
  • Analyzing protein adsorption and desorption behavior on the Si(p/n) surface using techniques like molecular dynamics simulations.
  • Assessing cell viability and function post-harvesting.

    • Main Results:

    • Cell sheets detached rapidly from the Si(p/n) surface within 10 minutes of visible-light exposure.
    • Cell viability and functions were maintained after the light-induced detachment process.
    • Preadsorbed proteins (e.g., BSA, collagen-I) exhibited light-induced desorption from the Si(p/n) surface.
    • Molecular dynamics simulations revealed that the photovoltaic effect of Si(p/n) under illumination generates long-range forces that trigger protein release, facilitating cell detachment.

    Conclusions:

    • Visible-light-induced cell sheet harvesting using Si(p/n) wafers is a promising technique for regenerative medicine.
    • The photovoltaic effect-mediated protein desorption mechanism is key to the efficient and gentle release of cell sheets.
    • This approach offers new insights into light-responsive biomaterials and protein-surface interactions.