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Updated: Feb 23, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Alternate synthesis to d-glycero-β-d-manno-heptose 1,7-biphosphate
Janelle Sauvageau1, Milan Bhasin1, Cynthia X Guo2
1Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, Ontario, K1A 0R6, Canada.
Abstract:
d-glycero-β-d-manno-heptose 1,7-biphosphate (HBP) is an enzymatic intermediate in the biosynthesis of the heptose component of lipopolysaccharide (LPS), and was recently revealed to be a pathogen-associated molecular pattern (PAMP) that allows detection of Gram-negative bacteria by the mammalian immune system. Cellular detection of HBP depends upon its stimulation of a cascade that leads to the phosphorylation and assembly of the TRAF-interacting with forkhead-associated domain protein A (TIFA), which activates the transcription factor NF-κB. In this note, an alternate chemical synthesis of HBP is described and its biological activity is established, providing pure material for further assessing and exploiting the biological activity of this compound.
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