Fertility: Store-Operated Ca²⁺ Entry in Germ Cells: Role in Egg Activation

At the time of fertilization, the sperm activates the egg and induces embryonic development by triggering an elevation in the egg's intracellular free Ca²⁺ concentration. In mammals the initial Ca²⁺ rise is followed by a series of repetitive Ca²⁺ transients (known as oscillations) that last for several hours. Although the source of Ca²⁺ during the signaling process is primarily the egg's smooth endoplasmic reticulum, the oscillations stop in the absence of extracellular Ca²⁺ indicating that a Ca²⁺ influx across the plasma membrane is essential to sustain them. Depletion of the intracellular stores using specific inhibitors generates a Ca²⁺ entry across the plasma membrane of eggs of various species, and a continuous influx of Ca²⁺ has been linked to the sperm-induced Ca²⁺ oscillations in the mouse; these data indicate that store-operated Ca²⁺ entry (SOCE) operates in eggs and may be the mechanism that maintains the long-lasting Ca²⁺ signal at fertilization. Recent findings suggest that the signaling proteins STIM1 and Orai1 are present in eggs; they are responsible for mediating SOCE, and their functions are essential for proper Ca²⁺ signaling at fertilization to support normal embryo development.