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Updated: Feb 22, 2026

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
Published on: November 26, 2015
Down for the count: The critical endpoint in ototoxicity remains the cytocochleogram
David V Gauvin1, Joshua Yoder1, Andrea Koch1
1MPI Research, 54943 North Main Street, Mattawan, MI 49071, USA.
Abstract:
There are three major assays that must be conducted in standard investigational new drug (IND) -enabling ototoxicity study designs: 1) functional acoustic threshold measurements (Auditory Brainstem Respsonse, ABR); 2) otohistopathology and 3) cytocochleograms. We provide evidence to demonstrate the unreliability of auditory threshold shifts (ABRs) to predict cochlear cell death and build a case for conducting full cochlea processing and cell count measurements from the complete cochlea from apex to base.
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