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Nuclear export factor 3 regulates localization of small nucleolar RNAs
Melissa W Li1, Arthur C Sletten1, Jiyeon Lee1
1Diabetes Research Center, Department of Medicine, St. Louis, Missouri 63110.
The Journal of Biological Chemistry
|October 13, 2017
Summary
Nuclear export factor 3 (NXF3) transports small nucleolar RNAs (snoRNAs) between cellular compartments. NXF3 regulates snoRNA distribution, impacting their function in response to cellular stress.
Area of Science:
- Molecular Biology
- RNA Biology
- Cell Biology
Background:
- Small nucleolar RNAs (snoRNAs) are crucial for RNA modification within the nucleus.
- Evidence suggests snoRNAs can be found in the cytoplasm, with their nucleocytoplasmic transport influenced by stress conditions.
Purpose of the Study:
- To identify factors regulating snoRNA nucleocytoplasmic transport.
- To elucidate the role of nuclear export factor 3 (NXF3) in snoRNA localization.
Main Methods:
- Genome-wide shRNA screen to identify transport factors.
- RNA-sequencing to analyze NXF3-snoRNA interactions.
- Gain- and loss-of-function experiments for NXF3 and NXF1.
- Forskolin treatment to assess stress-induced localization changes.
Main Results:
- NXF3 identified as a transporter of ribosomal protein L13a (Rpl13a) box C/D snoRNAs.
- NXF3 interacts with diverse box C/D and box H/ACA snoRNAs.
- NXF3, but not NXF1, modulates cytosolic Rpl13a snoRNA levels under homeostatic conditions.
- Forskolin-induced reduction of cytosolic snoRNAs depends on NXF3.
Conclusions:
- NXF3 plays a significant role in regulating the nucleocytoplasmic distribution of various snoRNAs.
- This regulation is linked to cellular stress responses and impacts snoRNA localization.
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