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Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining

Ayad Eddaoudi1, Stephanie Louise Canning2, Itaru Kato3

  • 1Flow Cytometry Core Facility, Camelia Botnar Laboratories, UCL Great Ormond Street Institute of Child Health, 85 Lamb's Conduit, London, WC1N 3JH, UK. a.eddaoudi@ucl.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2017
PubMed

Keywords:
Cell cycleG0Hoechst 33342Live-cell sorti ngMultiparametric flow cytometryPyronin Y

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...