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SERS-Fluorescence Dual-Mode pH-Sensing Method Based on Janus Microparticles.

Shuai Yue1, Xiaoting Sun1, Ning Wang1

  • 1Research Center for Analytical Sciences, Northeastern University , Shenyang 110819, P. R. China.

ACS Applied Materials & Interfaces
|October 25, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a dual-mode pH sensor using Janus microgels, combining surface-enhanced Raman scattering (SERS) and fluorescence for accurate measurements across a wide pH range, even in acidic conditions.

Keywords:
Janus microparticlesSERSdual-mode pH sensingextracellular pHfluorescencegastric juice pH

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Existing pH sensing methods have limitations, particularly in strong acidic media.
  • Dual-mode sensing offers enhanced specificity and imaging capabilities.

Purpose of the Study:

  • To develop a novel dual-mode pH-sensing method using Janus microgels.
  • To combine surface-enhanced Raman scattering (SERS) and fluorescence for pH detection.
  • To create a reliable sensor for a wide pH range, including acidic conditions.

Main Methods:

  • Fabrication of Janus microparticles with independent hemispheres using a centrifugal microfluidic chip.
  • Encapsulation of pH-dependent 4-mercaptobenzoic acid (SERS) and carbon dots (fluorescence) in separate hemispheres.
  • Utilizing the volumetric change of hydrogels in response to pH for sensing.

Main Results:

  • Successful development of a dual-mode SERS-fluorescence pH sensor.
  • Sensitive and reliable pH measurement in the range of 1.0 to 8.0 without interference between hemispheres.
  • Demonstrated validity for in vitro measurement of gastric juice and extracellular pH.
  • Microparticles showed good stability, reversibility, biocompatibility, and semipermeability.

Conclusions:

  • The developed Janus microgel sensor effectively overcomes limitations of SERS-only methods in acidic media.
  • The sensor demonstrates potential for in vivo continuous pH monitoring as implantable devices.
  • This dual-mode approach offers a promising platform for advanced biosensing applications.