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Updated: Feb 19, 2026

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Published on: April 16, 2014

Quantitative Live Cell FLIM Imaging in Three Dimensions.

Alix Le Marois¹, Klaus Suhling²

¹Department of Physics, King's College London, Strand, London, WC2R 2LS, UK.

Advances in Experimental Medicine and Biology

|October 29, 2017

Summary

This summary is machine-generated.

This chapter introduces fluorescence lifetime imaging microscopy (FLIM) for quantitative live cell analysis. It covers FLIM data recording, analysis, and applications in 3D tissue imaging and multiplexed detection.

Keywords:

FLIM data analysis FLIM techniques Multi-channel FLIM Multi-modal FLIM Optical sectioning Time-correlated single photon counting (TCSPC)

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Area of Science:

Biophotonics
Cellular Imaging
Microscopy

Background:

Fluorescence lifetime imaging microscopy (FLIM) offers unique insights into cellular environments.
Quantitative analysis of biological samples requires advanced imaging techniques.

Purpose of the Study:

Introduce the concept and utility of fluorescence lifetime in live cell imaging.
Detail methods for recording and analyzing FLIM data.
Explore applications of FLIM in 3D tissue and live cell imaging.

Main Methods:

Principles of fluorescence lifetime measurement.
Techniques for FLIM data acquisition.
Methods for FLIM data analysis and interpretation.

Main Results:

Demonstration of FLIM's quantitative capabilities in live cell studies.
Examples of FLIM application in complex biological systems.
Overview of multiplexed FLIM detection strategies.

Conclusions:

FLIM is a powerful tool for quantitative live cell and tissue imaging.
Understanding FLIM data analysis is crucial for biological research.
Advanced FLIM techniques enable deeper insights into cellular dynamics.