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Three-Dimensional Tissue Models and Available Probes for Multi-Parametric Live Cell Microscopy: A Brief Overview.
Neil O'Donnell1, Ruslan I Dmitriev2
1Metabolic Imaging Group, School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.
Advances in Experimental Medicine and Biology
|October 29, 2017
Summary
Novel 3D tissue models advance regenerative medicine but face imaging challenges. This review explores live cell microscopy compatibility and multi-parametric imaging probes for these complex models.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Cell Biology
Background:
- Recent advances in tissue engineering have introduced novel 3D tissue models.
- These models mimic in vivo cell and tissue organization, aiding research.
- Challenges remain in integrating 3D models with conventional cell biology techniques.
Purpose of the Study:
- To discuss the applicability of 3D tissue models for live cell microscopy.
- To review available fluorescent and phosphorescent probes for multi-parametric imaging in 3D models.
Main Methods:
- Literature review of 3D tissue models and live cell imaging techniques.
- Analysis of probe compatibility with 3D environments and microscopy modalities.
Main Results:
- 3D tissue models offer in vivo-like environments but present technical hurdles.
- Various live cell microscopy techniques can be adapted for 3D models.
- A range of fluorescent and phosphorescent probes enable multi-parametric analysis.
Conclusions:
- Overcoming challenges in 3D tissue model imaging is crucial for advancing regenerative medicine.
- The development and application of advanced imaging probes are key to realizing the full potential of 3D models.
