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Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

Open-source do-it-yourself multi-color fluorescence smartphone microscopy.

Yulung Sung¹, Fernando Campa¹, Wei-Chuan Shih^1,2,3,4

¹Department of Electrical & Computer Engineering, University of Houston, 4800 Calhoun Rd, Houston, TX 77204, USA.

Biomedical Optics Express

|December 1, 2017

Summary

This summary is machine-generated.

Researchers developed a simple, low-cost fluorescence smartphone microscope using a contact lens and guided illumination. This DIY fluorescence microscopy enables on-site analysis and point-of-care diagnostics for cellular and microbiological investigations.

Keywords:

(100.1455) Blind deconvolution (110.0180) Microscopy (120.3620) Lens system design (180.2520) Fluorescence microscopy (260.6970) Total internal reflection

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Area of Science:

Biomedical imaging
Microscopy
Mobile health technology

Background:

Fluorescence microscopy is crucial for cellular and microbiological studies.
Existing smartphone fluorescence microscopes are complex, limiting widespread use.
Need for accessible, portable fluorescence imaging solutions.

Purpose of the Study:

To develop a simple, cost-effective, and portable multi-color fluorescence smartphone microscope.
To enable on-site analysis, monitoring, and point-of-care diagnostics.
To overcome alignment challenges in current smartphone microscopy.

Main Methods:

Utilized a single contact lens as an add-on objective lens.
Implemented slide-launched total-internal-reflection guided illumination.
Tested on common fluorescence microscopy tasks: autofluorescence, fluorescent stains, and immunofluorescence.

Main Results:

Demonstrated a functional multi-color fluorescence smartphone microscope.
The design is open-source, simple, and cost-effective.
Successfully applied to autofluorescence, fluorescent stains, and immunofluorescence imaging.

Conclusions:

The developed smartphone microscope offers a viable DIY solution for fluorescence imaging.
Potential to significantly broaden the adoption of fluorescence microscopy in various settings.
Facilitates on-demand monitoring and point-of-care diagnostics through accessible technology.