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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Published on: October 3, 2014
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Multilineage Constructs for Scaffold-Based Tissue Engineering: A Review of Tissue-Specific Challenges
Timothée Baudequin1, Maryam Tabrizian1,2
1Faculty of Medicine, Biomat'X Laboratory, Department of Biomedical Engineering, McGill University, 740 ave. Dr. Penfield, Room 4300, Montréal, QC H3A 0G1, Québec, Canada.
Advanced Healthcare Materials
|December 2, 2017
Summary
This review explores using multiple cell types in tissue engineering to regenerate complex tissues. Coculturing diverse cells in scaffolds and controlled environments advances composite tissue development.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Cell Biology
Background:
- Interfacial tissue regeneration is complex due to varying biological, physical, and chemical properties.
- Developing in vitro composite tissues requires integrating distinct cell lineages.
- Existing research often focuses on single-tissue coculturing techniques.
Purpose of the Study:
- To review recent literature on using multiple cell types in coculture systems for tissue-engineered constructs.
- To explore strategies for mimicking native tissue interfaces and improving complex tissue substitutes.
- To differentiate from studies focused solely on technical aspects of single-tissue coculturing.
Main Methods:
- Literature review of cocultured tissue engineering studies.
- Analysis of variables including scaffolds, cells, and in vitro culture environments.
- Examination of tissue-specific coculture methods and approaches.
Main Results:
- Identification of key variables influencing cocultured tissue engineering outcomes.
- Overview of established coculture methods for various tissue types.
- Highlighting emerging trends and cross-disciplinary applications in coculture strategies.
Conclusions:
- Coculturing diverse cell lineages is crucial for advanced tissue engineering.
- Understanding tissue-specific challenges can drive innovation in interfacial tissue regeneration.
- This review provides a foundation for developing novel strategies for complex tissue substitutes.
