Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

775.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
775.3K
Next-generation Sequencing03:00

Next-generation Sequencing

98.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.0K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Potential Impact of Reduced Donor Funding for HIV Programs on Malawi's Disease Burden.

AIDS (London, England)·2026
Same author

Clinical Impact and Cost-Effectiveness of Improving Access to Cryptococcal Meningitis Diagnostics and Treatment in Malawi.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Dataset for rapid estimation of femoral neck loading during gait and dynamic exercises.

Scientific data·2026
Same author

Smoking, Diabetes, Workers' Compensation, and Traumatic Tears Are Risk Factors for Postoperative Shoulder Stiffness After Arthroscopic Rotator Cuff Repair.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2026
Same author

Estimating System-Wide Healthcare Costs Using a Health System Model: Application to the Thanzi La Onse Model of Malawi.

Applied health economics and health policy·2026
Same author

Nurse Transitions From Classroom to Clinical: Defining and Prioritizing Nursing Informatics Competencies.

Nursing education perspectives·2026
Same journal

Covalency control of photomagnetic relaxation in a manganese(II) photoswitch.

Nature chemistry·2026
Same journal

Trefoil polymers from a knotted synthon.

Nature chemistry·2026
Same journal

Inverted metal-free active template synthesis of rotaxanes via axle‑mediated macrocyclization.

Nature chemistry·2026
Same journal

Concise synthesis and strain-release diversification of bridgehead-substituted [2]-ladderanes.

Nature chemistry·2026
Same journal

Singlet fission mediates carotenoid-to-bacteriochlorophyll energy transfer in purple photosynthetic bacteria.

Nature chemistry·2026
Same journal

Programming stacking order in conducting van der Waals metal-organic frameworks through ligand aggregation.

Nature chemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 16, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.1K

Predicting DNA hybridization kinetics from sequence.

Jinny X Zhang1,2, John Z Fang1, Wei Duan1

  • 1Department of Bioengineering, Rice University, Houston, Texas 77030, USA.

Nature Chemistry
|December 20, 2017
PubMed
Summary
This summary is machine-generated.

Scientists developed a new algorithm to predict DNA hybridization rate constants using sequence information. This Weighted Neighbour Voting (WNV) model accurately forecasts hybridization kinetics, aiding in genomics research probe design.

More Related Videos

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.2K
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

7.3K

Related Experiment Videos

Last Updated: Feb 16, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.1K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.2K
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

7.3K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Bioinformatics

Background:

  • Hybridization is a fundamental molecular process in biology and biotechnology.
  • Current methods lack accurate predictive models for hybridization rate constants based solely on sequence information.

Purpose of the Study:

  • To develop a predictive model for determining DNA hybridization rate constants using sequence information.
  • To enable the design of efficient probe sequences for genomics research through accurate kinetic prediction.

Main Methods:

  • Performed 210 fluorescence kinetics experiments to study DNA hybridization kinetics.
  • Investigated 100 different DNA target and probe pairs (36-nt sub-sequences of CYCS and VEGF genes) across a temperature range (28-55°C).
  • Developed a Weighted Neighbour Voting (WNV) prediction algorithm with automated feature selection and weighting optimization.

Main Results:

  • The final six-feature WNV model predicts hybridization rate constants with approximately 91% accuracy.
  • The model predicts new sequences' hybridization rate constants within a factor of 3.
  • Leave-one-out cross-validation was used to assess model accuracy.

Conclusions:

  • The developed WNV algorithm provides an accurate method for predicting DNA hybridization rate constants from sequence data.
  • Accurate prediction of hybridization kinetics is crucial for designing effective probe sequences in genomics and biotechnology applications.