Related Experiment Video
Updated: Feb 15, 2026

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
Published on: August 9, 2011
A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy
C O S Sorzano1,2, J Vargas1, J Otón1
1Centro Nacional de Biotecnología, CSIC, c/Darwin 3, Cantoblanco, 28049 Madrid, Spain.
Abstract:
One of the key steps in Electron Microscopy is the tomographic reconstruction of a three-dimensional (3D) map of the specimen being studied from a set of two-dimensional (2D) projections acquired at the microscope. This tomographic reconstruction may be performed with different reconstruction algorithms that can be grouped into several large families: direct Fourier inversion methods, back-projection methods, Radon methods, or iterative algorithms. In this review, we focus on the latter family of algorithms, explaining the mathematical rationale behind the different algorithms in this family as they have been introduced in the field of Electron Microscopy. We cover their use in Single Particle Analysis (SPA) as well as in Electron Tomography (ET).
Related Concept Videos
Surveys
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Electron Microscopy
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Transmission Electron Microscopy
Immunogold Electron Microscopy

