Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimizing "self-wicking" nanowire grids.

Hui Wei1, Venkata P Dandey1, Zhening Zhang1

  • 1The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA.

Journal of Structural Biology
|January 11, 2018
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization and modulation of human insulin degrading enzyme conformational dynamics to control enzyme activity.

eLife·2026
Same author

Characterization Standard for <i>In-situ</i> Cryo-electron Tomography.

bioRxiv : the preprint server for biology·2026
Same author

AreTomoLive: automated reconstruction of comprehensively corrected and denoised cryo-electron tomograms in real time and at high throughput.

Nature methods·2026
Same author

copick: An open dataset interface and toolkit for collaborative annotation and analysis of cryo-electron tomography data.

Protein science : a publication of the Protein Society·2026
Same author

Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography.

IUCrJ·2026
Same author

A realistic phantom dataset for benchmarking cryo-ET data annotation.

Nature methods·2025
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
See all related articles

A novel self-blotting transmission electron microscopy (TEM) grid with nanowires simplifies sample preparation for cryo-electron microscopy (cryoEM). This innovation enhances thin film formation for improved imaging. Keywords: cryo-electron microscopy, TEM grid, nanowires, sample preparation.

Area of Science:

  • Materials Science
  • Biophysics
  • Microscopy

Background:

  • Cryo-electron microscopy (cryoEM) requires rapid vitrification of samples.
  • Traditional sample preparation can be challenging, impacting image quality.
  • Development of improved sample holders is crucial for cryoEM advancements.

Purpose of the Study:

  • To introduce a novel self-blotting transmission electron microscopy (TEM) grid.
  • To detail the fabrication and characterization of these nanowire-based grids.
  • To demonstrate their utility in sample preparation for cryoEM.

Main Methods:

  • Nanowires grown on copper TEM grids via a simple chemical reaction.
  • Utilizing the smooth side of the grid to adhere to holey substrates (e.g., carbon, gold).
Keywords:
Nanowire gridsSpotitonTEM gridscryoEM vitrification

Related Experiment Videos

  • Characterization of grid quality and behavior under various conditions.

    • Main Results:

    • The nanowire grids effectively wick away liquid, creating thin films.
    • Successful fabrication demonstrated using diverse fenestrated substrates.
    • Grids exhibit predictable behavior under tested conditions.

    Conclusions:

    • The self-blotting TEM grid offers a simplified approach to cryoEM sample preparation.
    • This method facilitates rapid liquid removal, crucial for vitrification.
    • The developed grids show promise for enhancing cryoEM imaging.