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Updated: Feb 15, 2026
14:53
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
Published on: May 13, 2014
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Transcriptional Profiling Using RNA-Seq to Study Hypoxia-Mediated Gene Regulation
Surendra K Shukla1, Ryan J King1, Pankaj K Singh2,3,4,5
1The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 14, 2018
Summary
Hypoxia alters cell physiology, regulated by hypoxia-inducible factors (HIFs). RNA sequencing (RNA-Seq) reveals global gene expression changes, offering insights into cellular responses to low oxygen environments.
Area of Science:
- Molecular Biology
- Cellular Physiology
- Genomics
Background:
- Hypoxic environments induce significant physiological and molecular changes in cells.
- Hypoxia-inducible factors (HIFs) are key transcription factors regulating cellular responses to low oxygen.
- HIF1, a heterodimer, controls gene expression related to cell growth, metabolism, and proliferation.
Purpose of the Study:
- To describe the comprehensive process of studying hypoxia-mediated gene expression using RNA-Seq.
- To elucidate cellular alterations in response to hypoxic conditions.
- To understand the regulation of gene expression by specific factors and environmental stimuli.
Main Methods:
- Hypoxic treatment of cells.
- RNA isolation and quality assessment.
- cDNA library preparation and quality control for RNA sequencing.
Main Results:
- RNA-Seq enables global analysis of gene expression patterns under hypoxia.
- This technique provides a comprehensive understanding of cellular alterations.
- Facilitates the study of gene regulation by specific factors and environmental stimuli.
Conclusions:
- RNA-Seq is a powerful tool for investigating hypoxia-induced gene expression changes.
- The described methodology provides a complete workflow for analyzing cellular responses to hypoxia.
- Understanding these responses is crucial for various biological and medical research areas.
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