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Updated: Feb 15, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

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Published on: May 20, 2022

Replication Domains: Genome Compartmentalization into Functional Replication Units.

Peiyao A Zhao¹, Juan Carlos Rivera-Mulia¹, David M Gilbert^2,3

¹Department of Biological Science, Florida State University, Tallahassee, FL, USA.

Advances in Experimental Medicine and Biology

|January 23, 2018

Summary

This summary is machine-generated.

DNA replication timing follows a program regulated during cell cycles and development. Replication domains (RDs), which are stable chromosome structures, switch timing between different cell states, impacting gene regulation.

Keywords:

Cell cycle Chromatin Nucleus Replication domain (RD)Replication foci Replication timing (RT)Topologically associating domain (TAD)

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Area of Science:

Genomics
Cell Biology
Epigenetics

Background:

DNA replication timing is crucial for cell cycle regulation, development, and differentiation.
Replication domains (RDs) are key regulatory units, often coinciding with topologically associating domains (TADs).
RDs and TADs represent stable chromosomal structures conserved across cell cycles and development.

Purpose of the Study:

To investigate the regulation and dynamics of the DNA replication timing programme.
To understand the relationship between replication domains (RDs), topologically associating domains (TADs), and chromosomal structure.
To explore how replication timing signatures change across distinct cell states during development and disease.

Main Methods:

Analysis of DNA replication timing patterns.
Chromatin conformation capture (3C) methods for mapping topologically associating domains (TADs).
Functional genomics approaches to study gene regulation and chromatin context.

Main Results:

Replication domains (RDs) are stable chromosomal units, often corresponding to TADs.
Approximately half of genomic RDs exhibit altered replication timing between different cell states.
Replication timing signatures are unique to distinct cell states during development and disease progression.

Conclusions:

Replication timing programmes are dynamic and cell-state specific, despite the stability of RD boundaries.
Understanding replication timing is key to deciphering its role in gene regulation and disease.
Advances in functional genomics will illuminate the causes and consequences of replication timing regulation.