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Updated: Feb 15, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Development and Validation of an Analytical Methodology Based on Liquid Chromatography-Electrospray Tandem Mass
Mustafa Cittan1,2, Ali Çelik3
1Department of Chemistry, Faculty of Science and Letters, Şehit Prof. Dr. İlhan Varank Campus, Manisa Celal Bayar University, 45140 Yunusemre, Manisa, Turkey.
Abstract:
A simple method was validated for the analysis of 31 phenolic compounds using liquid chromatography-electrospray tandem mass spectrometry. Proposed method was successfully applied to the determination of phenolic compounds in an olive leaf extract and 24 compounds were analyzed quantitatively. Olive biophenols were extracted from olive leaves by using microwave-assisted extraction with acceptable recovery values between 78.1 and 108.7%. Good linearities were obtained with correlation coefficients over 0.9916 from calibration curves of the phenolic compounds. The limits of quantifications were from 0.14 to 3.2 μg g-1. Intra-day and inter-day precision studies indicated that the proposed method was repeatable. As a result, it was confirmed that the proposed method was highly reliable for determination of the phenolic species in olive leaf extracts.
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