High temperature exposure did not affect induced 2n pollen viability in Populus

Area of Science:

• Plant genetics and breeding
• Reproductive biology
• Molecular cytology

Background:

• High temperature exposure is a common method to induce 2n gametes in Populus.
• The impact of high temperatures on the viability of induced 2n pollen is not well understood.

Purpose of the Study:

• To investigate the effect of high temperature exposure (38 and 41°C) on the viability of induced 2n pollen in Populus canescens.
• To assess pollen morphology, in vitro germination, and triploid production capacity of high-temperature-induced 2n pollen.

Main Methods:

• Observations of meiosis in Populus canescens.
• Induction of 2n pollen using high temperatures (38 and 41°C).
• Analysis of pollen morphology, in vitro germination rates, and successful triploid production via crossing.

Main Results:

• Meiotic stages and treatment duration significantly influenced the occurrence rate of induced 2n pollen.
• High temperatures led to decreased pollen production and increased abortion, with some effects on ectexine deposition.
• No significant difference in germination rate was found between natural and high-temperature-induced 2n pollen (26.7% vs. 26.2%).

Conclusions:

• High temperature exposure (38 and 41°C) does not lead to dysfunctional induced 2n pollen in Populus canescens.
• Induced 2n pollen retains viability and functionality, demonstrated by successful in vitro germination and triploid production.