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The sedative activity of flavonoids from Passiflora quadrangularis is mediated through the GABAergic pathway
Andressa Corneo Gazola1, Geison Modesti Costa2, Silvana Maria Zucolotto3
1Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, 88040-970, Brazil; Departamento de Farmácia, Centro Universitário Barriga Verde, Orleans, 88870-000 Brazil.
Abstract:
The aim of this study was to investigate the sedative activity of the aqueous leaf extract of Passiflora quadrangularis, a species that is widely cultivated and consumed in South America, and to identify its main constituents and elucidate the involvement of the GABAergic pathway in its mechanism of action. The bioguided fractionation of the crude extract showed a positive relationship between the sedative activity of the extract and its flavonoids. The methods employed to identify and isolate its main flavonoids resulted in the identification of vitexin-2''-O-xyloside, vitexin-2''-O-glucoside, orientin-2''-O-xyloside and orientin-2''-O-glucoside. Vitexin-2"-O-xyloside, the major flavonoid of the extract, showed sedative activity after oral administration in mice.
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