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Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Published on: August 29, 2022
Survival of Mesenchymal Stem Cells in Different Methods of Nebulization
A V Aver'yanov1, A G Konoplyannikov2, N S Antonov3
1Research Institute of Pulmonology, Federal Biomedical Agency, Moscow, Russia. averyanovav@mail.ru.
Abstract:
We compared survival of bone marrow mesenchymal stem cells after compressor, ultrasound, and mesh nebulization of the cell suspension over 10 min. Viability of stromal cells was best preserved after compressor nebulization (72%). Cell survival after ultrasonic nebulization was significantly lower (20%). After mesh nebulization, no live cells were found. Thus, compressor nebulization is the most preferable method of the production of cell aerosol for their delivery to the lower respiratory tract.
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