Dual-mode holographic microscopy imaging platform

Area of Science:

• Optics and Photonics
• Microscopy Technology
• Biomedical Imaging

Background:

• Lensless microscopy offers advantages in cost and portability.
• Achieving high resolution and large field of view simultaneously remains a challenge.
• Existing lensless techniques often compromise on imaging speed or quality.

Purpose of the Study:

• To develop a novel dual-mode lensless microscopy platform.
• To enable real-time imaging with switchable magnification and resolution.
• To enhance image quality through advanced processing techniques.

Main Methods:

• Utilized wavelength multiplexing for dual illumination with different wavefront curvatures (collimated and divergent).
• Integrated the MISHELF (Microscopy with Single Hologram Encoding and Light Field) microscopy concept for divergent illumination.
• Employed resolution test targets, flowing microbeads, and live sperm cells for performance validation.

Main Results:

• Demonstrated real-time dual-mode imaging with distinct magnification and resolution capabilities.
• Achieved a large field of view (FOV) with collimated illumination and micron-range resolution with divergent illumination.
• Showcased improved image quality via noise averaging and twin image minimization using MISHELF microscopy.

Conclusions:

• The proposed dual-mode lensless microscopy platform offers versatile imaging capabilities.
• This technology paves the way for next-generation lensless microscopes with enhanced performance.
• The system is suitable for imaging both static and dynamic complex biological samples.