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Updated: Feb 13, 2026

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
Published on: October 16, 2016
Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos
Wei Wang1, Claudia Racioppi1, Basile Gravez1
1Center for Developmental Genetics, Department of Biology, New York University, New York, NY, USA.
Abstract:
Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.
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