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Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
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Aligned laminin core-polydioxanone/collagen shell fiber matrices effective for neuritogenesis
Su-Jin Song1, Yong Cheol Shin2, Sung Eun Kim1
1Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea.
Scientific Reports
|April 5, 2018
Summary
Biomimetic scaffolds with aligned laminin core-polydioxanone/collagen shell fibers promote neural tissue regeneration. These advanced nerve guidance conduits offer topographical and biochemical cues for enhanced neurite outgrowth and neurogenic differentiation.
Area of Science:
- Biomaterials Science
- Neuroscience
- Tissue Engineering
Background:
- Severe nerve injuries present significant challenges for spontaneous regeneration.
- Existing strategies for nerve regeneration face technical hurdles in achieving optimal outcomes.
Purpose of the Study:
- To design and fabricate biomimetic matrices for enhanced neural tissue regeneration.
- To investigate the potential of aligned laminin core-polydioxanone/collagen shell (Lam-PDO/Col) fibers as biofunctional scaffolds.
Main Methods:
- Fabrication of aligned Lam-PDO/Col core-shell fibers using magnetic field-assisted electrospinning with a coaxial system.
- Assessment of laminin release kinetics from the fabricated fibers.
- Evaluation of cellular behaviors, including neurite outgrowth and neurogenic differentiation, of hippocampal neuronal cells on the matrices.
Main Results:
- Successful fabrication of aligned Lam-PDO/Col core-shell fibers was confirmed.
- Steady and continuous release of laminin from the fiber core was demonstrated.
- Significantly enhanced cellular behaviors, neurite outgrowth, and neurogenic differentiation were observed on the matrices.
Conclusions:
- The aligned Lam-PDO/Col core-shell fiber matrices provide effective topographical and biochemical cues for promoting neuritogenesis.
- These biomimetic scaffolds represent a promising approach for advancing neural tissue regeneration strategies.
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