Related Experiment Video

Updated: Feb 11, 2026

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
07:44

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

15.6K

In situ semi-quantitative assessment of single-cell viability by resonance Raman spectroscopy

Zhu Mao1, Zhuo Liu, Jin Yang

  • 1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, P. R. China. hanxiaoxia@jlu.edu.cn zhaob@mail.jlu.edu.cn.

Chemical Communications (Cambridge, England)
|May 3, 2018
PubMed

Insights

Researchers developed a new method using resonance Raman scattering to precisely measure single-cell viability. This technique enables detailed studies of cellular metabolism in individual cells, crucial for understanding anticancer drug effects.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Anticancer drug research increasingly focuses on cytotoxic effects.
  • Accurate assessment of single-cell viability is essential for these studies.
  • Existing methods may lack the required selectivity or resolution.

Purpose of the Study:

  • To develop and present a novel method for quantifying single-cell viability.
  • To achieve high selectivity in viability measurements.
  • To enable single-cell level analysis of cellular metabolism.

Main Methods:

  • Development of a novel quantification method.
  • Utilizing resonance Raman scattering (RRS) for high selectivity.
  • Application of the RRS method for single-cell analysis.

Main Results:

  • Successful development of a novel method for single-cell viability quantification.
  • Demonstration of high selectivity in the RRS-based method.
  • Establishment of a tool for single-cell metabolic studies.

Conclusions:

  • The developed resonance Raman scattering method is a powerful tool for assessing single-cell viability.
  • This technique facilitates in-depth studies of cellular metabolism at the single-cell level.
  • The method holds significant potential for advancing anticancer drug research.

More Related Videos

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.6K
Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

3.0K

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.8K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.4K
Resonance02:52

Resonance

The Lewis structure of a nitrite anion (NO2−) may actually be drawn in two different ways, distinguished by the locations of the N-O and N=O bonds.
66.0K
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
3.5K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.5K
Series Resonance01:17

Series Resonance

The RLC circuit impedance is defined as the ratio of the supply voltage to the circuit current. Resonance in such a circuit occurs when the imaginary part of this impedance equals zero. This specific condition means that the inductive reactance is exactly equal to the capacitive reactance. The frequency at which this happens is known as the resonant frequency. Mathematically, the resonant frequency is inversely proportional to the square root of the product of the inductance (L) and capacitance...
875