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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Feb 10, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

How alternative splicing affects membrane-trafficking dynamics.

R Eric Blue¹, Ennessa G Curry¹, Nichlas M Engels¹

¹Department of Cell Biology & Physiology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Journal of Cell Science

|May 18, 2018

Summary

This summary is machine-generated.

Alternative splicing fine-tunes membrane trafficking proteins for tissue specialization during development. Dysregulation of this process, involving clathrin and SNAREs, contributes to human diseases.

Keywords:

Alternative splicing Membrane dynamics RNA-binding proteins Trafficking

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Last Updated: Feb 10, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

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Published on: October 9, 2014

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Membrane-trafficking pathways are crucial for cellular functions in health and disease.
Understanding tissue-specific regulation and developmental fine-tuning of these pathways remains a challenge.
Alternative splicing is a key post-transcriptional mechanism regulating gene expression in development and tissues.

Purpose of the Study:

To review how alternative splicing impacts membrane-trafficking proteins and tissue specialization.
To examine the alterations in this regulation during human disease.
To highlight the role of RNA-binding proteins in this process.

Main Methods:

Literature review of recent discoveries on alternative splicing and membrane trafficking.
Analysis of studies focusing on clathrin, SNAREs, and BAR-domain proteins.
Examination of the role of RNA-binding proteins in splicing regulation.

Main Results:

Alternative splicing of clathrin, SNAREs, and BAR-domain proteins influences endocytosis, secretion, and membrane dynamics.
Alternative splicing plays a significant role in tissue specialization and during development.
Dysregulation of alternative splicing in membrane-trafficking proteins is implicated in human diseases.

Conclusions:

Alternative splicing is a critical molecular regulator of membrane-trafficking pathways.
Understanding this interplay is vital for comprehending tissue specialization and disease pathogenesis.
Future research should focus on the physiological relevance and therapeutic potential of targeting alternative splicing in membrane trafficking.