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Updated: Feb 9, 2026

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Published on: June 9, 2019
Metabolite extraction for high-throughput FTICR-MS-based metabolomics of grapevine leaves
Marisa Maia1,2,3, Filipa Monteiro1, Mónica Sebastiana1
1Biosystems & Integrative Sciences Institute (BioISI), Science Faculty of Lisbon University, 1749-016 Lisbon, Portugal.
Abstract:
In metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increase of biological studies. Direct infusion Fourier-transform ion cyclotron-resonance mass spectrometry (DI-FTICR-MS) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. In any metabolic profiling study, the establishment of an effective metabolite extraction protocol is paramount. We developed an improved metabolite extraction method, compatible with DI-FTICR-MS-based metabolomics, using grapevine leaves. This extraction protocol allowed the extraction of polar and non-polar compounds, covering all major classes found in plants and increasing metabolome coverage.
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