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Updated: Feb 8, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
O-Glycosylation Enabled by N-(Glycosyloxy)acetamides
Miao Liu1,2, Bo-Han Li2, De-Cai Xiong2,3
1National Research Center for Carbohydrate Synthesis , Jiangxi Normal University , Nanchang 330022 , China.
Abstract:
A novel glycosylation protocol has been established by using N-(glycosyloxy)acetamides as glycosyl donors. The N-oxyacetamide leaving group in donors could be rapidly activated in the presence of Cu(OTf)2 or SnCl4 under microwave irradiation. This glycosylation process afforded the coupled products in high yields, and the reaction enjoyed a broad substrate scope, even for disarmed donors and hindered acceptors. The easy availability of the donors, the high stability of N-(glycosyloxy)acetamides, and the small leaving group make this method very practical.
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