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Defence cascade in Verticillium-infected grafted tomato
Ross N Nazar1, Xin Xu1, Josefa Blaya Fernandez1
1a Department of Molecular and Cellular Biology , University of Guelph , Guelph , ON Canada.
Plant Signaling & Behavior
|June 26, 2018
Summary
The tomato Ve1-gene confers resistance to Verticillium wilt by modulating cell-specific defense proteins. However, this resistance effect is not systemic, as Ve1 function is not transferred to susceptible tissues in grafted plants.
Area of Science:
- Plant Pathology
- Molecular Plant Breeding
- Proteomics
Background:
- Tomato plants possess the Ve1-gene, which confers resistance to the vascular wilt pathogen Verticillium dahliae race 1.
- Susceptible tomato varieties (ve1 plants) lack this resistance and are prone to Verticillium wilt.
Purpose of the Study:
- To investigate the proteomic changes associated with Verticillium wilt in tomato.
- To determine the specific effect of the Ve1-gene on the defense/stress protein cascade during infection.
- To assess whether Ve1-gene function can be systemically transferred in grafted plants.
Main Methods:
- Utilized reciprocal grafting of resistant and susceptible tomato near-isolines.
- Employed label-free Liquid Chromatography-Mass Spectrometry (LC-MS) for proteomic analysis.
- Quantified fungal colonization to correlate with defense responses.
Main Results:
- Defense/stress protein responses are cell-specific and correlate with the level of fungal colonization.
- The Ve1-gene mitigates the defense response in resistant tissues.
- Ve1-gene-mediated resistance effects were not observed in susceptible tissues of grafted plants.
Conclusions:
- The Ve1-gene's resistance mechanism against Verticillium wilt is localized and cell-specific.
- Resistance is not transmissible to susceptible tissues through grafting.
- Understanding Ve1 gene function is crucial for developing resistant tomato cultivars.
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