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Published on: September 27, 2017
Skin Ceramide Profile in Children With Atopic Dermatitis
Chun-Ping Shen, Mu-Tong Zhao, Zhi-Xin Jia1
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Background:
Atopic dermatitis (AD) is a common disease, which involves a disruption of the skin barrier function. Skin ceramide (CER) composition, which plays crucial roles in maintaining the barrier function of the stratum corneum, is changed in patients with AD.
Objective:
The aim of this study was to identify and quantify skin CER subclasses in association with disease severity in pediatric patients with AD.
Methods:
Two hundred thirteen patients were entered into the observational study. We compared their CER profiles using normal-phase high-performance liquid chromatography coupled with dynamic multiple reaction monitoring mass spectrometry.
Results:
In total, 12 subclasses of CERs were identified. We found that 2 subclasses, that is, CER[AS] and CER[NS], were elevated (P = 0.007 and 0.012, respectively) and correlated with Severity Scoring of Atopic Dermatitis (P = 0.004 and 0.004, respectively).
Conclusions:
Skin CER abundances are changed in children with AD compared with control subjects.
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