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Automated Single-Cell Analysis and Isolation System: A Paradigm Shift in Cell Screening Methods for Bio-medicines
Kenji Tatematsu1, Shun'ichi Kuroda2
1Department of Biomolecular Science and Reaction, The Institute of Scientific and Industrial Research (ISIR), Osaka University, Ibaraki, Osaka, Japan.
Advances in Experimental Medicine and Biology
|June 27, 2018
Summary
A novel automated single-cell robot enables high-throughput screening of cells for biopharmaceutical development. This technology facilitates the isolation of cells with optimal properties, advancing drug discovery and cell-based breeding methods.
Area of Science:
- Biotechnology
- Cell Biology
- Bio-medicine Development
Background:
- Conventional cell sorting methods like fluorescence-activated cell sorting (FACS) have limitations in high-throughput screening.
- The need for advanced methods to isolate single cells with specific favorable properties from large cell populations is critical for biopharmaceutical research.
Purpose of the Study:
- To introduce and review an automated single-cell analysis and isolation system (single-cell robot).
- To compare the capabilities of the single-cell robot with conventional fluorescence-activated cell sorting (FACS).
- To highlight the potential applications of the single-cell robot in various fields of bio-medicine development.
Main Methods:
- Development of an automated robot for non-invasive single-cell isolation from arrays exceeding 10^5 cells.
- High-throughput screening of mammalian cells (e.g., Chinese hamster ovary (CHO) cells, hybridomas) for high bio-medicine secretion.
- Screening of stem cells (e.g., embryonic stem (ES) cells) for pluripotency using the single-cell robot.
- Application of the system for de novo agonist design using yeast cells expressing mammalian cytokine receptors (e.g., EGFR, SSTR5, IL5R).
- Analysis of olfactory sensory neuron and odorant interactions to understand olfactory receptor recognition.
Main Results:
- The single-cell robot can perform high-throughput screening for cells producing high levels of bio-medicines and stem cells with high pluripotency.
- The system enables rational screening for de novo agonist design.
- Comprehensive analysis of olfactory sensory neuron-odorant interactions is achievable.
- The robot facilitates the establishment of new cell screening methods for biopharmaceuticals.
Conclusions:
- The developed single-cell robot offers unique features for high-throughput screening and isolation of cells with desired properties.
- This technology supports advancements in cell-based breeding and the development of novel biopharmaceuticals.
- The system has broad applicability in drug discovery, regenerative medicine, and understanding sensory mechanisms.
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