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Updated: Feb 8, 2026

Imaging the Intracellular Trafficking of APP with Photoactivatable GFP
Published on: October 17, 2015
Charge designable and tunable GFP as a target pH-responsive carrier for intracellular functional protein delivery and
Shanfang Hu1, Xiaoye Chen, Chunyang Lei
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. huangyan.hnu@gmail.com niezhou.hnu@gmail.com.
Abstract:
A charge designable and tunable green fluorescent protein (GFP)-based protein delivery strategy was proposed. The acquired His29GFP selectively permeates the cell membrane at a target pH of 6.5 and escapes from the endosome efficiently. The delivered RNase A caused substantial mRNA degradation in HeLa cells, and proliferation inhibition in different cell lines and a 3D tumor model at pH 6.5.
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