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Immunophenotype evaluation of Non-Hodgkin's lymphomas
Robab Sheikhpour1, Fatemeh Pourhosseini2, Hossein Neamatzadeh3
1Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Medical Journal of the Islamic Republic of Iran
|June 29, 2018
Summary
Immunohistochemistry aids in diagnosing Non-Hodgkin's Lymphoma (NHLs). This study found CD45 and CD20 markers prevalent in B-cell NHLs, while CD3 was common in T-cell NHLs.
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- Non-Hodgkin's Lymphoma (NHL) is a diverse group of lymphoid malignancies.
- NHLs are broadly categorized into B-cell and T-cell subtypes.
- Immunophenotyping of biopsy samples is crucial for accurate NHL diagnosis.
Purpose of the Study:
- To evaluate the utility of immunohistochemistry in classifying B-cell and T-cell NHLs.
- To identify characteristic biomarker expression patterns in different NHL subtypes.
- To compare findings with existing literature, considering potential geographic and genetic influences.
Main Methods:
- Retrospective analysis of 77 patients diagnosed with B-cell or T-cell lymphoma.
- Utilized immunohistochemistry to detect specific cell surface markers: CD2, CD3, CD20, and CD45.
- Data collected from Shahid Sadoghi hospital between 2010 and 2013.
Main Results:
- B-cell lymphoma comprised the majority of cases (87.01%), with lymph nodes and stomach as common primary sites.
- CD45 and CD20 co-expression was observed in 91.04% of B-cell lymphoma patients.
- T-cell lymphoma accounted for 12.98% of cases, with skin being a frequent primary site, and all T-cell cases showed CD3 expression.
Conclusions:
- Immunohistochemistry is a vital tool for the classification and diagnosis of NHLs.
- Observed variations in CD marker expression and patient demographics may be attributed to regional genetic and ethnic factors.
- This study highlights the importance of immunophenotyping in understanding NHL heterogeneity.