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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Published on: September 20, 2019
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Mitotic live-cell imaging at different timescales
Anna-Maria Olziersky1, Chris A Smith2, Nigel Burroughs3
1Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland.
Methods in Cell Biology
|June 30, 2018
Summary
This study presents novel live-cell microscopy methods to track chromosome segregation during cell division. These techniques allow detailed analysis of chromosome dynamics from seconds to days, ensuring accurate genetic material transmission.
Area of Science:
- Cell Biology
- Genetics
- Microscopy
Background:
- Mitosis is crucial for accurate genetic material transmission.
- Live-cell fluorescence microscopy allows dynamic analysis of cellular processes.
- Understanding chromosome segregation dynamics is key to cell division research.
Purpose of the Study:
- To present methodologies for studying chromosome segregation at multiple timescales.
- To enable detailed analysis of mitotic spindle and chromosome dynamics.
- To provide tools for investigating cell population fate over multiple cell divisions.
Main Methods:
- Automated tracking of kinetochores for seconds-to-minutes timescale analysis.
- Utilizing novel DNA live dyes for several-hour chromosome segregation studies.
- Acquiring image sequences over several days to observe cell population dynamics.
Main Results:
- Demonstrated automated kinetochore tracking for high-resolution dynamic analysis.
- Validated new DNA live dyes for extended chromosome segregation studies.
- Successfully visualized cell population fates across multiple consecutive cell divisions.
Conclusions:
- Developed versatile live-cell microscopy techniques for comprehensive chromosome segregation analysis.
- These methods offer new insights into the fidelity of genetic transmission during mitosis.
- The presented methodologies are applicable across various cell lines and research questions.
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