[Comparison of lymphatic staining methods based on whole organ sections of laryngeal carcinoma]

Objective:To compare the diagnostic potential of HE staining, enzymatic histochemistry and immunohistochemistry in displaying lymphatic vessel of laryngeal carcinoma.Method：We recruited 3 patients who were pathologically diagnozed as laryngeal squamous cell carcinoma and were performed total laryngectomy in the First Affiliated Hospital of Shanxi Medical University from April to December 2016. According to the improved Kawamoto's Film Method, frozen specimens of whole laryngeal squamous cell carcinoma were made. Immunohistochemistry, using lymphatic endothelial cell specific marker D2-40, enzymatic histochemistry (5-nucleotidase) and HE staining were used to stain the frozen sections of laryngeal carcinoma. Then the staining results of lymphatic vessels in the specimens of laryngeal carcinoma were compared by the optical microscope. Result：The background of HE and D2-40 immunohistochemical staining were clear.5-Nase staining had a deeper background and more nonspecific staining. By immunohistochemistry,102（97.1%,102/105）blood vessels were identified, and 3(2.9%) were partly positive. While using 5-Nase,56（53,3%,56/105）blood vessels were identified. In 105 lumen structures that could not be clearly judged,95（90,5%）were positive by D2-40,while by 5-Nase staining,89（84.8%）were positive including a large number of glands.In addition, positive cells scattered in dots or clusters were observed in 5-Nase and D2-40 staining sections, but these cells or structures could not be identified by HE staining. Conclusion：D2-40 immunohistochemistry may have certain applied value in the study of lymphatic vessels associated with laryngeal carcinoma.