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Enzymatic synthesis of [beta-32P]ADP using adenylate kinase and [gamma-32P]ATP
Analytical Biochemistry
|September 1, 1985
Abstract:
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretical obtainable.