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Updated: Feb 6, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
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Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

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Immunohistochemistry of the circadian clock in mouse and human vascular tissues

Ciprian B Anea1, Ana M Merloiu1, David J R Fulton1

  • 1Department of Pharmacology & Toxicology, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA.

Vessel Plus
|August 14, 2018
PubMed

Insights

Circadian clock proteins show varied expression patterns in different blood vessel layers and tissues in mice and humans. This cell-specific localization may influence how the circadian clock signals within the cardiovascular system.

Area of Science:

  • Cardiovascular Biology
  • Chronobiology
  • Molecular Biology

Background:

  • The body's physiological rhythms are regulated by the circadian clock, a molecular network.
  • Circadian clock components influence vascular remodeling, blood pressure, and signaling in blood vessels.

Purpose of the Study:

  • To determine the expression patterns of circadian clock proteins.
  • To analyze protein localization in the endothelium, smooth muscle, and adventitia of human and mouse vasculature.

Main Methods:

  • Immunohistochemistry was used on mouse aorta, carotid artery, femoral artery, lung, and heart sections at 12 AM and 12 PM.
  • Expression of clock components like Bmal1, Clock, Npas2, and Per was assessed.
  • Human saphenous vein samples were analyzed using immunoblotting and immunohistochemistry.

Main Results:

  • Bmal1, Clock, Npas, Per1, and Cry1 expression varied temporally and spatially across different mouse vasculature and tissues.
  • The human saphenous vein demonstrated clock gene expression, with Bmal1 and Cry showing oscillatory patterns via immunoblotting.

Conclusions:

  • Circadian clock components exhibit distinct expression and localization profiles within the cardiovascular system.
  • Cell-specific expression of these components may lead to nuanced circadian clock signaling in a cell-specific manner.
Abstract

Keywords:
Bmal1Circadian blood vesselClockCryPeraortahumanmousesmooth musclevascular endothelium

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