Related Experiment Video
Updated: Feb 6, 2026
12:22
Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
Published on: August 4, 2018
9.0K
Fidelity test for through-focus or volumetric type of optical imaging methods
Optics Express
|August 17, 2018
Summary
This study introduces a new method to assess the accuracy of through-focus (TF) optical imaging data. This protocol ensures reliable quantitative results from volumetric imaging in biological applications.
Area of Science:
- Optical Imaging
- Biophotonics
- Microscopy
Background:
- Through-focus (TF) or volumetric optical imaging is increasingly used in biology.
- Standardized protocols are crucial for quantitative imaging but lack for TF methods.
- Existing protocols are for conventional microscopy, not volumetric imaging.
Purpose of the Study:
- To present a novel method for assessing the fidelity of TF optical data.
- To establish rigorous testing protocols for TF optical imaging.
- To enable standardization and optimization of TF image acquisition.
Main Methods:
- Utilized TF scanning optical microscopy images.
- Developed a method to determine data fidelity.
- Applied the method to assess TF optical data quality.
Main Results:
- Presented a functional method for TF data fidelity determination.
- Demonstrated the applicability of the method using TF scanning microscopy.
- Laid groundwork for standardized TF imaging protocols.
Conclusions:
- A reliable method for assessing TF optical data fidelity is now available.
- This work addresses the need for rigorous testing protocols in TF imaging.
- Enables more accurate and quantitative volumetric optical microscopy.
Related Concept Videos
01:26Methods of Documentation IV: Focus Charting
1.7K
Focus Charting, also known as the focus charting system or "focus documentation," is a systematic documentation approach used in healthcare to organize patient information in medical records.
It typically involves three columns for recording information:
It typically involves three columns for recording information:
1.7K
01:18Sampling Methods: Sample Types
3.3K
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
3.3K
01:18Imaging Biological Samples with Optical Microscopy
10.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
10.6K
01:08Titrimetric Methods: Types and Commonly Used Strategies
2.5K
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
2.5K
01:16Focusing of Light in the Eye
6.0K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
6.0K
01:27Coping Strategies: Problem Focused
518
Coping strategies are methods people use to manage, tolerate, or reduce the effects of stressors. These strategies involve both behavioral and psychological actions to handle stressful situations. One common approach is problem-focused coping, which aims to change or eliminate the source of stress rather than merely addressing its consequences. This method involves taking direct action to resolve the issue causing stress.
For example, consider a student who struggles to understand their...
For example, consider a student who struggles to understand their...
518