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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Accreditation and Quality Assurance for Clinical Liquid Chromatography-Mass Spectrometry Laboratories
1Department of Laboratory Medicine, Clinical Laboratory, Zuckerberg San Francisco General Hospital, University of California San Francisco, 1001 Potrero Avenue. Building. 5 2M, San Francisco, CA 94110, USA.
Abstract:
For mass spectrometry (MS) testing in the clinical laboratory, postimplementation monitoring for quality is just as important as method development and validation but often receives less attention. Quality-assurance monitoring for liquid chromatography-tandem MS (LC-MS/MS) testing should be proactive rather than reactive and should monitor the entire testing process. An LC-MS/MS quality-assurance plan should cover overall batch review parameters, individual peak review parameters, system and reagent changes, and assessment of long-term accuracy. This article discusses Clinical Laboratory Improvement Amendments' regulations as they apply to LC-MS/MS-based testing and reviews available guidelines for LC-MS/MS quality assurance and postimplementation monitoring.
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