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Updated: Feb 6, 2026
05:59
An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules
Published on: November 4, 2022
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[Simultaneous Determination of Four Bioactive Components in Chrysanthemi Flos by Quantitative Analysis of
Summary
Quantitative analysis of multi-components by single marker (QAMS) accurately determines key compounds in Chrysanthemi Flos. This method is feasible for quality control, using chlorogenic acid as a reference marker.
Area of Science:
- Pharmacognosy
- Analytical Chemistry
- Phytochemistry
Background:
- Chrysanthemi Flos is a traditional medicinal herb.
- Ensuring the quality of herbal medicines is crucial.
- Standardized quality control methods are needed for Chrysanthemi Flos.
Purpose of the Study:
- To develop a simultaneous determination method for chlorogenic acid, galuteolin, quercetin, and acacetin in Chrysanthemi Flos.
- To validate the application of quantitative analysis of multi-components by single marker (QAMS) for quality control.
- To establish the reliability of using chlorogenic acid as a reference marker.
Main Methods:
- High-Performance Liquid Chromatography (HPLC) with a Shim-pack C18 column.
- Gradient elution using acetonitrile and phosphoric acid aqueous solution.
- Quantitative analysis of multi-components by single marker (QAMS) with chlorogenic acid as the reference standard.
Main Results:
- Relative correction factors (RCF) for galuteolin, quercetin, and acacetin relative to chlorogenic acid were determined.
- The RCF values showed low relative standard deviations (RSD), indicating method precision.
- QAMS results were statistically comparable to the external standard method, demonstrating accuracy.
Conclusions:
- The developed QAMS method is accurate and feasible for the simultaneous determination of target compounds.
- This method provides a reliable approach for the quality control of Chrysanthemi Flos.
- Chlorogenic acid serves as an effective single marker for evaluating the quality of galuteolin, quercetin, and acacetin in Chrysanthemi Flos.
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