Integrated contrast-transfer-function for aberration-corrected phase-contrast STEM
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Updated: Feb 5, 2026

Phase Contrast and Differential Interference Contrast DIC Microscopy
Published on: August 6, 2008
Takehito Seki1, Naoto Takanashi2, Eiji Abe2
1Institute of Engineering Innovation, University of Tokyo, Tokyo 113-8656, Japan.
Abstract:
We describe the optical conditions that are essentially necessary for phase-contrast imaging with aberration-corrected scanning transmission electron microscopy (STEM), whose depth of field has reached almost comparable to the specimen thickness. For such state-of-the-art STEM, contrast-transfer-function (CTF) should be defined not solely for the projected potential but multiply for each wavefront during the beam propagation across the specimen thickness; an integration of multiple CTFs (iCTF). We show that the iCTF concept explains fairly well characteristic annular-bright-field (ABF) imaging behaviors of heavy/light atom sites against the defocus changes, and also provide notable concerns on possible artifacts that arise from different imaging-depth dependences between the heavy/light atom sites.
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