Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

Characteristics of Practical Op Amps

Characteristics of Practical Op Amps

A difference amplifier, a crucial component in numerous electronic devices, ideally amplifies only the difference-mode signal, which is the difference between two input signals. However, in practical circuits, the output voltage depends on both the differential gain and the common-mode gain.
The ratio of differential gain to the common-mode gain is defined as the common-mode rejection ratio (CMRR). This ratio quantifies the ability of operational amplifiers (op-amps) to reject common-mode...

Theoretical Foundations of Nursing Practice

Theoretical Foundations of Nursing Practice

Theories play an essential role in organizing patient care. Theories refer to a proposed or followed belief, policy, or procedure that is the basis for action. Nursing theories are knowledge-based concepts that guide nurses' actions, influence nursing education and practice, and allow nurses to care for their patients.
Theories provide a perspective to assess patients' conditions and organize data and methods. They also assist in analyzing and interpreting information. They represent a...

Equivalent Circuits for Practical Transformers

Equivalent Circuits for Practical Transformers

The practical equivalent circuits of single-phase two-winding transformers exhibit significant deviations from their idealized versions due to the inherent properties of winding resistance and finite core permeability. These properties result in real and reactive power losses, affecting the transformer's performance. Understanding these deviations is crucial for designing more efficient transformers.
In a practical transformer, each winding exhibits resistance and leakage reactance. The...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Depth Perception and Spatial Vision

Depth Perception and Spatial Vision

Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrated bioinformatic multi-omics analysis and experimental verification reveal the oncogenic role of WDHD1 and its underlying mechanism in gastric cancer progression.

BMC cancer·2026

Same author

Integrated volatile and non-volatile metabolomics reveal aroma chemotypes in Capsicum cultivars.

Food chemistry: X·2026

Same author

Current status and progress of validation studies on the ICOPE scale: a scoping review.

Frontiers in public health·2026

Same author

In-situ Damascus-patterning enables tunable surface electric fields for bioactive titanium implants.

Bioactive materials·2026

Same author

<i>Gentiana tingnongiana</i> and <i>G. shangwui</i>, two new species of <i>Gentiana</i> (Gentianaceae) from the eastern Qinghai-Tibet Plateau, Sichuan province, China.

PhytoKeys·2026

Same author

Application Value of a nomogram integrating contrast-enhanced CT radiomics and clinical indicators in evaluating lymph node metastasis in pediatric peripheral neuroblastoma.

Cancer imaging : the official publication of the International Cancer Imaging Society·2026

Search research articles

Related Experiment Video

Updated: Feb 5, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

A practical wide-field Raman imaging method with high spectral and spatial resolution.

Haibo Li¹, Wenhua Luo², Gan Li¹

¹Science and Technology on Surface Physics and Chemistry Laboratory, Mianyang 621907, People's Republic of China.

The Review of Scientific Instruments

|September 7, 2018

Summary

A new wide-field Raman imaging platform enhances speed, sensitivity, and resolution for analyzing complex materials. This advanced technique offers detailed chemical mapping at the microscale, improving material characterization.

More Related Videos

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Published on: May 10, 2012

Related Experiment Videos

Last Updated: Feb 5, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Published on: May 10, 2012

Area of Science:

Spectroscopy
Chemical Imaging
Materials Science

Background:

Raman imaging is crucial for characterizing inhomogeneous systems.
Existing wide-field Raman imaging systems face limitations in speed, sensitivity, and resolution.

Purpose of the Study:

To develop a practical wide-field Raman imaging platform with enhanced performance.
To improve imaging speed, sensitivity, and spatial/spectral resolution for detailed chemical analysis.

Main Methods:

An inverted architecture integrating a tunable laser and wavelength-fixed filters was employed.
High-transmission fixed filters and a high-power tunable laser were utilized.
The system achieved spectral resolution of 1.5 cm⁻¹ and spatial resolution of 490 nm.

Main Results:

Imaging sensitivity was improved 5-10 times compared to systems using liquid-crystal tunable filters.
Raman images were acquired in minutes with easily tunable Raman shifts.
High spectral and spatial resolution provided detailed chemical fingerprint information.

Conclusions:

The developed Raman imaging platform offers significant improvements in speed, sensitivity, and resolution.
This method enables visualization of chemical spatial variations at the microscale.
The platform is expected to find broad applications in scientific research, particularly in materials science and chemical analysis.