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A simple fluorescent aptamer based assay coupled with fluorescence scanning capillary array for aflatoxin B1.
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. qiangzhao@rcees.ac.cn.
We created a simple aptamer fluorescence assay for detecting aflatoxin B1 (AFB1). This rapid, high-throughput method uses capillary arrays and shows promise for food safety and environmental monitoring.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Biosensors
Background:
- Aflatoxin B1 (AFB1) is a toxic contaminant found in food.
- Accurate and rapid detection methods for AFB1 are crucial for food safety.
Purpose of the Study:
- To develop a simple, rapid, and high-throughput aptamer-based fluorescence assay for AFB1 detection.
Main Methods:
- Utilized a 34-nucleotide aptamer labeled with fluorescein (FAM).
- Employed a fluorescence scanning capillary array for sample analysis.
- Investigated the fluorescence response upon AFB1 binding.
Main Results:
- AFB1 binding induced a significant increase in fluorescence.
- The assay demonstrated simplicity, rapidity, and high throughput with low sample consumption.
- Achieved a detection limit of 0.5 nM for AFB1.
Conclusions:
- The developed aptamer fluorescence assay is effective for AFB1 detection.
- The capillary array system offers advantages for multiple sample analysis.
- This method holds significant potential for food safety and environmental monitoring applications.
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