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Glaucoma is an eye condition characterized by increased intraocular pressure that damages the retina and optic nerve, leading to irreversible blindness if left untreated. The human eye has various components, including the cornea, iris, pupil, lens, and optic nerve. Aqueous humor is secreted by the epithelium of the ciliary body in the posterior chamber and flows through the trabecular meshwork and canal of Schlemm, maintaining normal intraocular pressure. The trabecular meshwork and the canal...
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Related Experiment Video

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Assessing Early Stage Open-Angle Glaucoma in Patients by Isolated-Check Visual Evoked Potential
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COMPARISON OF SPECTROSCOPIC PROPERTIES OF INTRAOCULAR FLUID IN PATIENTS WITH CATARACT AND PRIMARY OPEN-ANGLE

S V Kolotilov, V O Melnyk, A S Lytvynenko

    Fiziolohichnyi Zhurnal (Kiev, Ukraine : 1994)
    |September 12, 2018
    PubMed
    Summary
    This summary is machine-generated.

    Spectroscopic analysis of aqueous humor revealed higher absorbance at 202 nm in patients with primary open angle glaucoma (POAG) and cataract compared to those with cataract alone. This suggests metabolite accumulation in POAG patients, potentially due to reduced aqueous humor flow.

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    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Spectroscopy

    Background:

    • Cataract and primary open angle glaucoma (POAG) are common ocular conditions.
    • Aqueous humor composition can be altered in ocular diseases.
    • Spectroscopic analysis offers a method to investigate biochemical changes in aqueous humor.

    Purpose of the Study:

    • To compare the spectroscopic properties of aqueous humor in patients with cataract versus those with a combination of cataract and POAG.
    • To identify potential spectroscopic markers associated with POAG in aqueous humor.

    Main Methods:

    • Aqueous humor samples were collected from 60 cataract patients and 36 patients with cataract and POAG during surgery.
    • Absorption spectra were measured in the 190-1100 nm range.
    • Spectra were analyzed for absorption bands and absorbance values at specific wavelengths.

    Main Results:

    • Two main absorption bands were observed at 202 nm and 267 nm.
    • Mean absorbance at 202 nm was significantly lower in cataract patients without POAG compared to those with POAG.
    • No significant difference in mean absorbance at 267 nm was found between the groups.

    Conclusions:

    • Elevated aqueous humor absorbance at 202 nm in POAG patients may indicate metabolite accumulation.
    • This accumulation could be linked to decelerated aqueous humor flow in POAG.
    • Spectroscopic analysis of aqueous humor shows potential for differentiating ocular disease states.